MALDI-Based Mass Spectrometry in Clinical Testing: Focus on Bacterial Identification

The term “proteome” refers to the total of all proteins expressed in an organism. “proteomics” field research that includes not only information on expression levels individual proteins, but also their higher-order structures, intermolecular interactions, and post-translational modifications. core technology, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), is available for protein analysis thanks work Koichi Tanaka John Fenn, who were awarded Nobel Prize Chemistry 2002. most successful proteome clinical practice rapid microbial identification. This method determines bacterial species by comparing profile bacteria obtained desorption ionization-time flight MS (MALDI-TOF MS) with a database. superior simplicity, speed, accuracy classic speciation staining phenotyping. In microbiology, has had large impact diagnosis treatment infectious disease. Early disease are important, identification MALDI-TOF made major contribution this field.